The objective of this study was to characterize urban road dust particles and to study their possible health effects. Road re-suspended dust has been recognized as one of the major contributors to TSP elevating concentrations in Thessaloniki. Eight samples of road dust were collected from the accumulated matter at the edges of major roads in the historic centre of the city of Thessaloniki. The predominant size fraction, according to mass, was 125–500μm, while the mass fraction of the suspendable dust particles (20-63μm and <20μm) was the lowest. Special emphasis was given to the mineralogical characteristics of the urban deposits. Road dusts were mainly composed of quartz, calcite, while plagioclase, dolomite, Kfeldspars, amphiboles, micas and chlorite were contained in minor amounts. Amorphous phase was also determined mainly in the finer fractions (20-63μm and <20μm). Scanning electron microscopy shows that dust particles consist of subhedral to anhedral crystalline grains, near-spherical and irregular agglomerates as well as few organic materials. EDS analyses reveal that the composition of dust particles is basically Ca-rich, Fe-rich and silicates.